Coding

Part:BBa_K1676103

Designed by: Ooi Kean Hean   Group: iGEM15_NTU-Singapore   (2015-09-18)

Lactate Permease of E. Coli

This part is the Lactate Permease gene of E. Coli. It's function is to transport lactate into the cytoplasm. Previous studies have compared it's efficiency when cloned into Shewanella oneidensis MR1. However, it was found that the homolog from Pseudomonas aeruginosa enhances lactate consumption and growth of our chasis more than this gene.

More information on the characterisation of this gene can be found in this summary. Guides on the legend of the graphs can be found at the last page.

https://static.igem.org/mediawiki/2015/5/58/Prescreening_of_enzymes.pdf

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 48
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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